The study examined the effect of supervision support on health worker performance at Kilembe Hospital, Kasese District, Uganda. The study adopted a cross-sectional survey research design with mixed methods approach. A sample size of 152 respondents was considered for the study, 130 respondents participated, giving a response rate of 85.5%. The content validity index was calculated from, the questionnaire and it was 0.94 while the computed Cronbach’s alpha coefficient is 0.922 which is above 0.7 considered indicative of acceptable internal consistency therefore the questionnaire was deemed reliable. For qualitative data, to ensure consistency the KII and documentary review were guided by the research objective and question to keep the study focused. The study discovered that supervisors are sometimes not accessible at Kilembe hospital. The study therefore, recommends the need for Ministry of health to recruit more health workers since the study discovered workload as one of the major reasons where supervisors are sometimes not accessible.