The study aimed at the impact of commercialisation of fishing on gender roles, among lake Victoria shore fishing communities of uganda and it was guided by the following objectives; To examine the influence of culture on women and men’s participation in the commercialised fisheries sector, to find out how the commercialising of the fisheries sector has affected the traditional gender roles in the household and the wider community and to analyse the benefits derived by men and women from the commercialised fisheries. The study employed qualitative and quantitative research approaches/models. The qualitative approach was used to complement quantitative methods as a way of obtaining in depth information beyond what an ordinary survey could elicit. The study population involved fishermen, Beach Management Unit (BMU), local leaders and cultural leaders. Their views represent the views of the target population. Fishermen were selected because they are major stakeholders in the day to day running of the fishing activities in Katosi, therefore, they were in position to share their experiences. The sample size of the study involved (80) respondents of which (10) of them were the Beach Management Unit officials, (15) local leaders, and 55 were the local fishing communities. From the findings, most of the female in Katosi (12%) named inadequate capital as the most important limitation to men. For Katosi, culture was cited as the second most important constraint named by 20% of all women and 3% of the men respondents, reduced catch was named as the third most important constraint (11%). Revelations from in-depth interviews indicated that the Katosi theft situation is complicated and allegations of thieves disappearing to the nearby islands were rife. There is need to revive and promote cooperative fishing and marketing and therefore, cooperatives need to be promoted not only for socio-economic expediency but also for the fact that they can promote collective bargaining and provide useful channels for government to propagate ideas to the grassroots.