The purpose of the study was to investigate the role of entrepreneurship studies on the enterprise development in Itojo Sub County in Kisoro District. The study was based on findings from 325 respondents out of the sample size of 370 respondents who presented accurate data in relation to the objectives of the study. The objectives of the study were to examine whether developed enterprises are based on entrepreneurship studies, to identify the different entrepreneurship skills that can lead to success and finally to identify entrepreneurs who learnt entrepreneurship studies. Data was collected from the entrepreneurs and entrepreneurship consultants in Itojo Sub County in Kisoro District. According to the findings of the study, majority of the respondents strongly disagreed with a view that successful enterprises based on entrepreneurship studies. The findings of the study also show that the regression model successfully forecasts the variable that was the dependent variable. The regression model's statistical significance was denoted by F=114.231 and P=0.000, both less than 0.05. The disparity between the regression's initial mean square value of 8.607 and the remainder's mean square value of 0.075 serves as more evidence for this. This demonstrated the relationship between social economic development and entrepreneurship studies reductions. Upon the recommendations, there is a need to sensitize all the entrepreneurs in the community to adopt to learning entrepreneurship skills in order to make them understand business ethics.