This study aimed to evaluate the role of safety regulations in preventing workplace accidents at Fine Spinners Uganda Ltd., focusing on the impact of employee awareness, the implementation of safety measures, and the enforcement of safety regulations. The research employed a quantitative design, utilizing both primary and secondary data collected through structured questionnaires and company accident records. A purposive sample of 100 employees was selected from various departments, and descriptive statistics, Chi-square tests, paired sample t-tests, and regression analysis were applied to analyze the data. The results showed that while employee awareness of safety regulations was high, it had no significant impact on reducing workplace accidents. This finding highlighted that awareness alone was insufficient to prevent accidents, as suggested by previous research in the field. However, the study found a significant reduction in accidents after the implementation of safety regulations, indicating that proper enforcement and structured safety measures played a more critical role in accident prevention. The regression analysis further confirmed that stronger enforcement of safety regulations led to a notable decrease in accidents, emphasizing the importance of consistent regulatory practices. The findings were consistent with the literature, which emphasized the significance of enforcement and a strong safety culture in reducing workplace injuries. The study concluded that while awareness programs are important, the most effective strategy to prevent accidents is the implementation and rigorous enforcement of safety regulations. Based on these findings, the study recommended that Fine Spinners Uganda Ltd. provide regular safety training, enforce safety regulations consistently, and foster a safety-oriented culture within the organization to further reduce accidents and improve workplace safety. These recommendations aim to contribute to the broader understanding of workplace safety in Uganda’s textile industry, providing insights for both practitioners and policymakers to enhance occupational health and safety practices in the country’s