The major objective of this study lied in finding out whether there was a relationship between Pollution and Organisms Extinction in Kisoro Municipality, Kisoro District. Other objectives included: To establish the level of Pollution in Kisoro Municipality, Kisoro District and to find out the extent of Organisms Extinction in Kisoro Municipality, Kisoro District. Using Solvin's method, eighty participants from the Kisoro Municipality and Kisoro District were chosen for the size of the sample being studied. The study utilized a descriptive and observational research design. Microsoft Excel was used to analyze the data, and graphs, distributions of frequencies tables, and pie charts were used to illustrate the results. The study's data were analyzed using SPSS software. According to the results, there was an extremely favorable correlation between pollution and the disappearance of organisms (r = 0.994), and since the sig. value (.000) was much lower than the threshold level of importance required to declare a significant connection between pollution and extermination of organisms (0.01), this meant that pollution had a substantial effect on extinction of organisms. Since the P-value (0.00) was less than 0.05, this was statistically noteworthy. As a result, we reject the idea of a null hypothesis and come to the conclusion that there was no connection between contamination and organism extinction in the Kisoro Municipality. These pollutants disrupt the life cycles of living things, such as earthworms as there and they also kill, mutate, and produce adaptive electromagnetic radiation in some research.miu.ac.ug/publications 876 METROPOLITAN JOURNAL OF ENVIRONMENTAL AND HEALTH RESEARCH ISSN: 1813-4270 Vol. 2 Issue 3, June - 2023, Pages: 876-893 species, which leads to the extinction of the parent species. The researcher advised the government to establish well-guarded wildlife zones (game parks) in order to protect and preserve these species of plants and animals.